Frequency Domain Fluorimetry Using a Mercury Vapor Lamp
Frequency Domain (FD) fluorimetry, capitalizes on the frequency response function of a fluorophore and offers independence from light scatter and excitation/emission intensity variations in order to extract the sample's fluorescent lifetime. Mercury vapor lamps, a common source of industrial facility lighting, emit radiation that overlaps the UV/blue absorption spectrum of many fluorophores and may be used as an efficient and portable excitation source.
Abstract excerpt © SPIE
Journal of Applied Remote Sensing
Matthew J. Bohn, Michael A. Lundin, and Michael A. Marciniak "Frequency domain fluorimetry using a mercury vapor lamp," Journal of Applied Remote Sensing 3(1), 033524 (1 April 2009). https://doi.org/10.1117/1.3117448