Date of Award
3-2022
Document Type
Thesis
Degree Name
Master of Science
Department
Department of Systems Engineering and Management
First Advisor
Jeremy R. Slagley, PhD
Abstract
During a mass casualty medical evacuation after a bioaerosol attack, a decontamination method is needed that is effective at both decontamination and preventing the secondary hazard of biological particles reaerosolizing from contaminated clothing. However, neither the efficacy of current decontamination methods nor the risk of biological particle reaerosolization is significantly explored in existing literature. The goals of this thesis were to develop a repeatable methodology to quantify the reaerosolization of a biological contaminate off Airman Battle Uniform (ABU) fabric swatches, and to test the efficacy of one decontamination method (high-volume, low-pressure water) using 1 mpolystyrene latex (PSL) spheres as a surrogate. Four major methodologies were developed: Contamination using a Collison Nebulizer; Reaerosolization using a laboratory mixer and collection using an air pump and inhalable air sampler with a polyvinyl chloride (PVC) filter; Decontamination using a gravity-fed water shower; and Quantification using ultraviolet (UV) microscopy techniques via both human eye and computer techniques. All results for control samples showed little to no presence of PSL sphere-like particles, while the experimental trials showed a ~73 reduction in reaerosolization before and after decontamination via water, at the 99 confidence level (p-value = 0.0081), along with a change in deposition patterns from aerosol-like (before decontamination) to droplet-like (after decontamination).
AFIT Designator
AFIT-ENV-MS-22-M-187
DTIC Accession Number
AD1173768
Recommended Citation
Cooksey, George D., "Development of a Methodology for the Quantification of Reaerosolization of a Biological Contaminant Surrogate Particle from Military Uniform Fabric" (2022). Theses and Dissertations. 5391.
https://scholar.afit.edu/etd/5391
Included in
Environmental Health and Protection Commons, Environmental Microbiology and Microbial Ecology Commons